Table 2.

Comparison of two common scRNA-seq platforms for droplet microfluidics and split-pool barcoding: 10× Chromium and  sci-RNA-seq3

Parameter10× Chromium (Per Lane)sci-RNA-seq3 (Per Experiment)
Throughput<104 cells106 cells
Costs per cella$0.2$0.01
Sequencing depth required (per cell)>30,000 raw reads>5,000 raw reads
Number of detected genes per cell>2,000>500
Labor to generate libraryModerate (1–2 days)High (1–2 weeks)
Data preprocessing methodsUser-friendly software (e.g., CellRanger)Customized pipeline required
  • a Estimated costs for reagents are presented. Illumina sequencing costs are not included.