Table 1.

Histopathology and immunohistochemistry findings in kidneys from 4-methylumbelliferone–treated and control mice (n=5 and 7 respectively)

Findings4-MethylumbelliferoneControl P value
Glomeruli
 Mesangial expansion score0.94±0.21 (approximately 24% of glomeruli affected)1.24±0.16 (approximately 31% of glomeruli affected)0.017
 Glomerular injury index1.19±0.29 (approximately 30% of glomerular tuft area affected)1.53±0.23 (approximately 38% of glomerular tuft area affected)0.047
 % of segmental glomerulosclerosis9.0±1.814.9±7.50.090
 % of nodular glomerulosclerosis (Kimmelstiel-Wilson lesion)3.0 (0.0–13.0)1.0 (0.0–8.0)0.87
 % of global glomerulosclerosis6.2 (5.9–10.2)7.6 (2.6–20.0)0.76
 % of affected glomeruli54.4±15.164.6±10.00.19
 % of mesangiolysis5.6±2.47.7±4.90.41
 Glomerular diameter (µm)92.8±5.596.2±5.70.33
Vascular/Interstitium
 Severe arteriolar hyalinosisNot observed42.9% (3/7)0.20
 % of interstitial fibrosis and tubular atrophy6.7±3.96.3±3.50.85
Inflammation
 Nephritis and tubulitisNot observed28.6% (2/7)0.47
 CD68+ cell count – glomeruli1.0 (0.5–4.1)7.6 (2.4–21.0)0.081
 CD68+ cell count – interstitium2.0 (1.1–6.3)3.8 (1.1–74.2)0.71
 CD44+ cell count – glomeruli27.0 (19.9–46.4)34.1 (22.3–61.0)0.57
 CD44+ cell count – interstitium100.0 (74.6–115.9)138.5 (100.5–254.8)0.18
  • 4-MU, 4-methylumbelliferone.